线虫是一种寄生类病害,难以防治,严重抑制植物生长。温度是影响线虫侵染能力的主要因素之一,但作用机理尚不明确。为探讨线虫抗性机制,以抗、感线虫品种(N、C)为对象,用人工接种,分析3个温度下不同品种根系抗氧化酶活性、组织结构变化规律、抗性基因表达特征。受线虫侵染,不同抗性品种根POD、CAT活性及糖、HRGP含量增加,SOD活性降低,N的POD、CAT活性及糖、HRGP含量高于C。同时,2品种的抗氧化酶活性变幅在25℃最大,35℃最小。不同温度下,N受线虫侵染后根系组织结构无巨型细胞、卵囊,但C出现大量巨型细胞。在25℃,C根系组织巨型细胞数量比15℃、35℃多,而N的木质化程度在25℃较15℃、35℃加重。在不同温度,未接种线虫的N的根系RK基因表达量在各时期无差异。线虫侵染促进N的RK基因上调,表达量在不同温度下在侵染2
d达峰值,25℃、15℃的表达量是35℃的3.36、1.62倍。线虫侵染使感病品种根SOD活性、渗透调节物质含量降低,且木质部出现大量巨型细胞,从而感病。抗病品种在线虫侵染下有较强的ROS清除能力、渗透调节能力,根组织结构无明显变化,RK基因上调表达,利于抵御线虫侵染。与25℃相比,烟在35℃通过提升抗氧化酶活性、保持良好组织结构降低线虫侵染;RK基因在35℃未失活仅表达量下降,仍对线虫侵染产生抑制。
Nematodes are parasitic pests that are difficult to control and severely inhibit plant
growth. Temperature is one of the main factors affecting nematode infectivity, but the
underlying mechanism of action remains unclear. To explore the nematode resistance
mechanism, the antioxidant enzyme activity, tissue structure changes, and resistance gene
(Rk) expression characteristics of the roots of different cultivars at three temperatures
were analyzed by the artificial inoculation of nematode-resistant and susceptible cultivars
(hereafter referred to as N and C). The activity of peroxidase (POD) and catalase (CAT) and
the content of sugar and hydroxyproline-rich glycoprotein (HRGP) in the roots of different
resistant cultivars increased after nematode infection, while the activity of superoxide
dismutase (SOD) decreased. Notably, the activity of POD and CAT and the content of sugar and
HRGP in N were higher than those in C. Meanwhile, the activity of antioxidant enzymes in
both cultivars varied the most at 25°C and the least at 35°C. There were no giant cells and
oocysts in the root tissue structure of N after nematode infection at different
temperatures, while a large number of giant cells appeared in C. The number of giant cells
in the root tissue of C at 25°C was more than that at 15°C or 35°C, while the degree of
lignification in N was more severe at 25°C than that at 15°C or 35°C. In addition, there was
no difference in the expression of the Rk gene at different temperatures in the roots of N
uninoculated with nematodes, whereas the up-regulated expression of the Rk gene in N was
promoted after nematode infection. The expression levels peaked on the 2nd day after
nematode infection at different temperatures, and the expression levels at 25°C and 15°C
were 3.36 and 1.62 times higher than that at 35°C. The activity of SOD and the content of
osmoregulatory substances reduced in the roots of C after nematode infection, and a large
number of giant cells appeared in the xylem, resulting in susceptibility. N showed strong
reactive oxygen species (ROS) scavenging ability and osmoregulatory ability, no significant
changes in root tissue structure, and the up-regulated expression of the Rk gene after
nematode infection, which were conducive to resisting nematode infection. Compared with the
situation at 25°C, the nematode infection of tobacco was lowered by increasing the activity
of antioxidant enzymes and maintaining good tissue structure at 35°C, and the expression of
the Rk gene was not inactivated but decreased at 35°C, which still inhibited nematode
infection.